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Literature summary extracted from

  • Brim, R.L.; Nance, M.R.; Youngstrom, D.W.; Narasimhan, D.; Zhan, C.G.; Tesmer, J.J.; Sunahara, R.K.; Woods, J.H.
    A thermally stable form of bacterial cocaine esterase: a potential therapeutic agent for treatment of cocaine abuse (2010), Mol. Pharmacol., 77, 593-600.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.1.84 medicine the wild type cocaine esterase cannot be used as a pharmacotherapy for cocaine abuse because of its 13.7-min half-life at 37°C Rhodococcus sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.84 mutant enzyme L169K/G173Q, hanging drop vapor diffusion method, using 1.7 M ammonium sulfate, 10 mM Tris-HCl, pH 7.3, and 25 mM NaCl Rhodococcus sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.84 L169K/G173Q highly thermostable mutant with a half-life of 2.9 days at 37°C Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.84 0.0072
-
 cocaine wild type enzyme, without preincubation, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 0.0073
-
 cocaine wild type enzyme, after 24 h preincubation at 37°C, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 0.0229
-
 cocaine mutant enzyme L169K/G173Q, after 24 h preincubation at 37°C, at pH 7.4 and 37°C Rhodococcus sp.
3.1.1.84 0.0303
-
 cocaine mutant enzyme L169K/G173Q, without preincubation, at pH 7.4 and 37°C Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.84 Rhodococcus sp. Q9L9D7
-
-
3.1.1.84 Rhodococcus sp. MB1 Q9L9D7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.84 cocaine + H2O
-
 Rhodococcus sp. ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 cocaine + H2O
-
 Rhodococcus sp. MB1 ecgonine methyl ester + benzoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.84 cocE
-
 Rhodococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.84 37
-
 the wild type enzyme shows 10.8% residual activity after 24 h at 37°C, mutant enzyme L169K/G173Q shows 77.5% residual activity after 24 h at 37°C Rhodococcus sp.